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Microfibrillar structure of type I collagen in situ

机译:I型胶原原位微纤维结构

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摘要

The fibrous collagens are ubiquitous in animals and form the structural basis of all mammalian connective tissues, including those of the heart, vasculature, skin, cornea, bones, and tendons. However, in comparison with what is known of their production, turnover and physiological structure, very little is understood regarding the three-dimensional arrangement of collagen molecules in naturally occurring fibrils. This knowledge may provide insight into key biological processes such as fibrillo-genesis and tissue remodeling and into diseases such as heart disease and cancer. Here we present a crystallographic determination of the collagen type I supermolecular structure, where the molecular conformation of each collagen segment found within the naturally occurring crystallographic unit cell has been defined (P1, a ≈ 40.0 Å, b ≈ 27.0 Å, c ≈ 678 Å, α ≈ 89.2°, β ≈ 94.6°, γ ≈ 105.6°; reflections: 414, overlapping, 232, and nonoverlapping, 182; resolution, 5.16 Å axial and 11.1 Å equatorial). This structure shows that the molecular packing topology of the collagen molecule is such that packing neighbors are arranged to form a supertwisted (discontinuous) right-handed microfibril that interdigitates with neighboring microfibrils. This interdigitation establishes the crystallographic superlattice, which is formed of quasihexagonally packed collagen molecules. In addition, the molecular packing structure of collagen shown here provides information concerning the potential modes of action of two prominent molecules involved in human health and disease: decorin and the Matrix Metallo-Proteinase (MMP) collagenase.
机译:纤维状胶原在动物中无处不在,并构成所有哺乳动物结缔组织的结构基础,包括心脏,脉管系统,皮肤,角膜,骨头和肌腱的结缔组织。然而,与其已知的生产,更新和生理结构相比,关于天然存在的原纤维中胶原分子的三维排列了解得很少。这些知识可以提供对诸如纤维形成和组织重塑之类的关键生物学过程以及对诸如心脏病和癌症之类的疾病的见识。在这里,我们介绍了I型胶原超分子结构的晶体学测定,其中定义了在天然晶体学晶胞中发现的每个胶原片段的分子构象(P1,a≈40.0Å,b≈27.0Å,c≈678Å ,α≈89.2°,β≈94.6°,γ≈105.6°;反射率:414,重叠232,不重叠182;分辨率,轴向5.16Å,赤道11.1。该结构表明,胶原分子的分子堆积拓扑使得堆积邻居被布置成形成与邻近的微纤维相互交叉的超扭曲(不连续)的右旋微纤维。这种交叉指形建立了由准六边形堆积的胶原分子形成的晶体学超晶格。此外,此处显示的胶原蛋白的分子堆积结构提供了有关涉及人类健康和疾病的两个重要分子的潜在作用方式的信息:核心蛋白聚糖和基质金属蛋白酶(MMP)胶原酶。

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